PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 51
... ssDNA for the next 5-10 cycles ( Figure 3 ) . Figure 4 shows the accumulation of dsDNA and ssDNA during a typical amplification of a genomic sequence ( a 242 - bp fragment of the HLA - DQa locus ) , using an initial ratio of 50 pmol of ...
... ssDNA for the next 5-10 cycles ( Figure 3 ) . Figure 4 shows the accumulation of dsDNA and ssDNA during a typical amplification of a genomic sequence ( a 242 - bp fragment of the HLA - DQa locus ) , using an initial ratio of 50 pmol of ...
Page 53
... ssDNA . The ssDNA generated can then be sequenced using either the PCR primer that is limiting or an internal primer and applying conventional protocols for incorporation sequencing or labelled primer sequencing . The population of ssDNA ...
... ssDNA . The ssDNA generated can then be sequenced using either the PCR primer that is limiting or an internal primer and applying conventional protocols for incorporation sequencing or labelled primer sequencing . The population of ssDNA ...
Page 55
... ssDNA PROBE BY PCR As an example of the use of asymmetric PCR for generating radioactively- labeled ssDNA as hybridization probe , the central region of the second exon of ... SSDNA abcdef radioactive labeled Figure 5. Generation of ssDNA.
... ssDNA PROBE BY PCR As an example of the use of asymmetric PCR for generating radioactively- labeled ssDNA as hybridization probe , the central region of the second exon of ... SSDNA abcdef radioactive labeled Figure 5. Generation of ssDNA.
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube