PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 24
... step , at temperatures above 37 ° C , and 3 ) a primer extension step , usually at temperatures between 65 ° C and 80 ° C , with an optimum centered around 72 ° C . Now the temperatures for the annealing and extension steps could be ...
... step , at temperatures above 37 ° C , and 3 ) a primer extension step , usually at temperatures between 65 ° C and 80 ° C , with an optimum centered around 72 ° C . Now the temperatures for the annealing and extension steps could be ...
Page 37
... step 4 ( use 1 % Laureth 1Z instead of NP40 and Tween 20 and Proteinase K at 200 μg / ml ) . Proceed from there through step 7 . Protocol D. Plucked Hairs 1. Cut off 0.5 cm of freshly plucked hair at root end . Use fine - tipped forceps ...
... step 4 ( use 1 % Laureth 1Z instead of NP40 and Tween 20 and Proteinase K at 200 μg / ml ) . Proceed from there through step 7 . Protocol D. Plucked Hairs 1. Cut off 0.5 cm of freshly plucked hair at root end . Use fine - tipped forceps ...
Page 81
... step in the reaction may allow the primers to amplify additional regions in genomic DNA other than the target segment . It is likely that the primers are not perfectly matched to these additional target sequences , but after an initial ...
... step in the reaction may allow the primers to amplify additional regions in genomic DNA other than the target segment . It is likely that the primers are not perfectly matched to these additional target sequences , but after an initial ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube