PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page 18
... synthesis is seen at very high temperatures ( > 90 ° C ) . DNA synthesis at higher temperatures in vitro may be limited by the stability of the primer or priming - strand and the template - strand duplex . Although Taq DNA polymerase ...
... synthesis is seen at very high temperatures ( > 90 ° C ) . DNA synthesis at higher temperatures in vitro may be limited by the stability of the primer or priming - strand and the template - strand duplex . Although Taq DNA polymerase ...
Page 19
... synthesis - dependent , strand replacement , 5 ' - 3'- exonuclease activity . There is little , if any , degradation of a 5 ' 32P - labeled oligodeoxynucleotide , either as single - stranded DNA or where annealed to an M13 template ...
... synthesis - dependent , strand replacement , 5 ' - 3'- exonuclease activity . There is little , if any , degradation of a 5 ' 32P - labeled oligodeoxynucleotide , either as single - stranded DNA or where annealed to an M13 template ...
Page 21
... synthesis at a replication fork ? If only limited synthesis occurs under such conditions , are there Thermus " helicases " which facilitate efficient displacement synthesis ? The ability to catalyze processive , displacement synthesis ...
... synthesis at a replication fork ? If only limited synthesis occurs under such conditions , are there Thermus " helicases " which facilitate efficient displacement synthesis ? The ability to catalyze processive , displacement synthesis ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube