PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page 2
... target sequence in the genomic template . " This effect was revealed by experiments in which normal genomic DNA ... target fragments were produced . Thus , in the absence of the " right " template , the " wrong " sequences became ...
... target sequence in the genomic template . " This effect was revealed by experiments in which normal genomic DNA ... target fragments were produced . Thus , in the absence of the " right " template , the " wrong " sequences became ...
Page 64
... sequence . Oligonucleotide primers are represented by the arrows adjacent to their annealing sites in the target DNA sequence . The two middle or " inside " primers , which anneal to the same segment of the target DNA but to the ...
... sequence . Oligonucleotide primers are represented by the arrows adjacent to their annealing sites in the target DNA sequence . The two middle or " inside " primers , which anneal to the same segment of the target DNA but to the ...
Page 65
... target DNA net deletion complementary insertion insertion motet promoter complementary coding sequence complementary & promoter complementary coding sequence complementary coding sequence 363636363639999998 aromatera34353635 ...
... target DNA net deletion complementary insertion insertion motet promoter complementary coding sequence complementary & promoter complementary coding sequence complementary coding sequence 363636363639999998 aromatera34353635 ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
PCR Amplification of Specific Sequences from | 9 |
Taq DNA Polymerase | 17 |
Copyright | |
13 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated B-globin cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand gene genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube