PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page 14
... temperatures helps maintain maximum polymerase activity throughout the reaction . The temperature at which annealing is done depends on the length and GC content of the primers . A temperature of 55 ° C is a good starting point for ...
... temperatures helps maintain maximum polymerase activity throughout the reaction . The temperature at which annealing is done depends on the length and GC content of the primers . A temperature of 55 ° C is a good starting point for ...
Page 24
... temperature cycling , but no suitable devices existed at the time PCR was invented to meet this need . Cetus Instrument Systems modified a Pro / Pette TM liquid handler ( the Perkin- Elmer Cetus multi - channel automated liquid handler ) ...
... temperature cycling , but no suitable devices existed at the time PCR was invented to meet this need . Cetus Instrument Systems modified a Pro / Pette TM liquid handler ( the Perkin- Elmer Cetus multi - channel automated liquid handler ) ...
Page 29
... temperature change is somewhat delayed vis - a - vis the block temperature change . The result of using this method of temperature control is that temperature overshoots , which could prevent amplification , and undershoots , which ...
... temperature change is somewhat delayed vis - a - vis the block temperature change . The result of using this method of temperature control is that temperature overshoots , which could prevent amplification , and undershoots , which ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube