PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page 2
... ( template , primers , Taq polymerase , dNTP's , and buffer ) could all be assembled and the amplification reaction carried out by simply cycling the temperature within the reaction tube . The effect of varying the reaction parameters ...
... ( template , primers , Taq polymerase , dNTP's , and buffer ) could all be assembled and the amplification reaction carried out by simply cycling the temperature within the reaction tube . The effect of varying the reaction parameters ...
Page 4
... template by the Taq polymerase.10 Although different template sequences may have a somewhat different " mutability " and different reaction conditions may influence the fidelity of the Taq polymerase , the original " high " error rate ...
... template by the Taq polymerase.10 Although different template sequences may have a somewhat different " mutability " and different reaction conditions may influence the fidelity of the Taq polymerase , the original " high " error rate ...
Page 50
... template complex from extending . Strand reassociation will permit only a fraction of the templates to participate in the sequencing reaction , resulting in weak sequencing ladders . To reduce this problem , either a variant of the ...
... template complex from extending . Strand reassociation will permit only a fraction of the templates to participate in the sequencing reaction , resulting in weak sequencing ladders . To reduce this problem , either a variant of the ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube