Physical Principles and Techniques of Protein Chemistry Part A, Part 1Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of maps from X-ray methods and the diffraction data from fibrous proteins. The subsequent chapters cover discussions on UV spectroscopy of proteins; luminescence properties of proteins and related compounds; and perturbation and flow methods for evaluation of proteins’ dynamic properties and rate constants. Other chapters deal with the evaluation of proteins’ dielectric properties using dielectric relaxation, electric birefringence, and dichroism techniques. The concluding chapters outline the theoretical and experimental advances of the electrophoretic and gel filtration methods for the study of protein structure and molecular weight. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
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Page vii
... light of current theories. These volumes may be considered as complementary to recent volumes on preparative and analytical protein chemistry such as “Methods in Enzymology,” Volume XI (C. H. W. Hirs, ed.) and to specialized treatises ...
... light of current theories. These volumes may be considered as complementary to recent volumes on preparative and analytical protein chemistry such as “Methods in Enzymology,” Volume XI (C. H. W. Hirs, ed.) and to specialized treatises ...
Page xiii
... Light Scattering Serge N. Timashefi and Robert Townend Low Angle X-Ray Scattering O. Kratky Infrared Methods R. D. B. Fraser and E. Suzuki Optical Rotatory Dispersion and Circular Dichroism J. Brahms Magnetic Resonance Spectroscopy ...
... Light Scattering Serge N. Timashefi and Robert Townend Low Angle X-Ray Scattering O. Kratky Infrared Methods R. D. B. Fraser and E. Suzuki Optical Rotatory Dispersion and Circular Dichroism J. Brahms Magnetic Resonance Spectroscopy ...
Page 3
... light microscope, in which light rays are replaced by a beam of accelerated electrons. Electrons produced by thermal emission from a tungsten filament are accelerated through voltages Of the order of 100 kV, forming the illuminating ...
... light microscope, in which light rays are replaced by a beam of accelerated electrons. Electrons produced by thermal emission from a tungsten filament are accelerated through voltages Of the order of 100 kV, forming the illuminating ...
Page 4
... light microscopes; manufacturers' literature must be consulted concerning the operation of any given make of instrument. A schematic comparison of the optical components of light and electron microscopes is given in Fig. 1. 2. Factors ...
... light microscopes; manufacturers' literature must be consulted concerning the operation of any given make of instrument. A schematic comparison of the optical components of light and electron microscopes is given in Fig. 1. 2. Factors ...
Page 5
... Light microscope (b) Electron microscope. FIG. 1. Comparison of the optical components of (a), the compound light microscope and (b), the electron microscope. As shown, each instrument consists of a source (lamp, electron gun) ...
... Light microscope (b) Electron microscope. FIG. 1. Comparison of the optical components of (a), the compound light microscope and (b), the electron microscope. As shown, each instrument consists of a source (lamp, electron gun) ...
Contents
59 | |
Chapter 3 Ultraviolet Absorption | 101 |
Chapter 4 Fluorescence of Proteins | 171 |
Chapter 5 Perturbation and Flow Techniques | 245 |
Chapter 6 Dielectric Properties of Proteins I Dielectric Relaxation | 291 |
Chapter 7 Dielectric Properties of Proteins II Electric Birefringence and Dichroism | 335 |
Chapter 8 Electrophoresis | 369 |
Chapter 9 Analytical Gel Filtration | 451 |
Author Index | 497 |
Subject Index | 509 |
Common terms and phrases
absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient concentration curve defined denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction diffusion dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission energy enzyme equation equilibrium excitation experimental factor field strength film filters first flow fluorescence fraction frequency gel filtration groups intensity interactions ionic strength ions light macromolecules magnification measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic phenylalanine photomultiplier Phys plot polarization polymer protein quantum yield ratio reaction reflections relaxation residues ribonuclease rotation shown in Fig significant solution solvent specific specimen spectra structure sufficiently technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone