Physical Principles and Techniques of Protein Chemistry Part A, Part 1Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of maps from X-ray methods and the diffraction data from fibrous proteins. The subsequent chapters cover discussions on UV spectroscopy of proteins; luminescence properties of proteins and related compounds; and perturbation and flow methods for evaluation of proteins’ dynamic properties and rate constants. Other chapters deal with the evaluation of proteins’ dielectric properties using dielectric relaxation, electric birefringence, and dichroism techniques. The concluding chapters outline the theoretical and experimental advances of the electrophoretic and gel filtration methods for the study of protein structure and molecular weight. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
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Page 60
... reflection; coordinates of a reciprocal lattice point. h is also used for the axial rise per unit in describing ... reflection with indices hkl F (hlcl) structure factor for the reflection with indices hlrl [F (kid) 1 structure amplitude ...
... reflection; coordinates of a reciprocal lattice point. h is also used for the axial rise per unit in describing ... reflection with indices hkl F (hlcl) structure factor for the reflection with indices hlrl [F (kid) 1 structure amplitude ...
Page 62
... reflections, as they are called, would be solely determined by the nature of the lattice oea'“ cathfle Monochromatic ... reflection persists will be small. If the number of molecules is decreased, this range will increase in proportion ...
... reflections, as they are called, would be solely determined by the nature of the lattice oea'“ cathfle Monochromatic ... reflection persists will be small. If the number of molecules is decreased, this range will increase in proportion ...
Page 67
... reflections obtained from the crystal. The space group is usually quoted in a symbolic form, for example C2 and P43212, which are shorthand descriptions of the symmetry of the internal structure of the crystal in terms of the type of ...
... reflections obtained from the crystal. The space group is usually quoted in a symbolic form, for example C2 and P43212, which are shorthand descriptions of the symmetry of the internal structure of the crystal in terms of the type of ...
Page 68
... reflections, if recorded with a suitable camera (Fig. 7), lie FIG. 7. X-Ray diffraction pattern taken down the 4-fold axis of a tetragonal crystal of hen egg lysozyme using a precession camera (courtesy of Dr. C. C. F. Blake). The array ...
... reflections, if recorded with a suitable camera (Fig. 7), lie FIG. 7. X-Ray diffraction pattern taken down the 4-fold axis of a tetragonal crystal of hen egg lysozyme using a precession camera (courtesy of Dr. C. C. F. Blake). The array ...
Page 69
... reflection and its position in the reciprocal lattice is described by three integers h, It, and l as shown in Fig. 8. The FIG. 8. Terminology used in describing positions in the reciprocal lattice. The symbol 311 indicates the lattice ...
... reflection and its position in the reciprocal lattice is described by three integers h, It, and l as shown in Fig. 8. The FIG. 8. Terminology used in describing positions in the reciprocal lattice. The symbol 311 indicates the lattice ...
Contents
59 | |
Chapter 3 Ultraviolet Absorption | 101 |
Chapter 4 Fluorescence of Proteins | 171 |
Chapter 5 Perturbation and Flow Techniques | 245 |
Chapter 6 Dielectric Properties of Proteins I Dielectric Relaxation | 291 |
Chapter 7 Dielectric Properties of Proteins II Electric Birefringence and Dichroism | 335 |
Chapter 8 Electrophoresis | 369 |
Chapter 9 Analytical Gel Filtration | 451 |
Author Index | 497 |
Subject Index | 509 |
Common terms and phrases
absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient concentration curve defined denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction diffusion dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission energy enzyme equation equilibrium excitation experimental factor field strength film filters first flow fluorescence fraction frequency gel filtration groups intensity interactions ionic strength ions light macromolecules magnification measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic phenylalanine photomultiplier Phys plot polarization polymer protein quantum yield ratio reaction reflections relaxation residues ribonuclease rotation shown in Fig significant solution solvent specific specimen spectra structure sufficiently technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone