Physical Principles and Techniques of Protein Chemistry Part A, Part 1Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of maps from X-ray methods and the diffraction data from fibrous proteins. The subsequent chapters cover discussions on UV spectroscopy of proteins; luminescence properties of proteins and related compounds; and perturbation and flow methods for evaluation of proteins’ dynamic properties and rate constants. Other chapters deal with the evaluation of proteins’ dielectric properties using dielectric relaxation, electric birefringence, and dichroism techniques. The concluding chapters outline the theoretical and experimental advances of the electrophoretic and gel filtration methods for the study of protein structure and molecular weight. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
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Results 1-5 of 30
Page 59
... Unit Cell B. Symmetry Axes C. The Space Group . D. The Asymmetric Unit . IV. The Reciprocal Lattice V. The Structure Factor VI. Fourier Synthesis . . . A. Sections and Projections B. Resolving Power . . . . C. Thermal Vibrations and ...
... Unit Cell B. Symmetry Axes C. The Space Group . D. The Asymmetric Unit . IV. The Reciprocal Lattice V. The Structure Factor VI. Fourier Synthesis . . . A. Sections and Projections B. Resolving Power . . . . C. Thermal Vibrations and ...
Page 60
... unit cell 23,-, y,, z,- the fractional coordinates of the jib atom in the unit cell h, k, l indices of a reflection; coordinates of a reciprocal lattice point. h is also used for the axial rise per unit in describing helical structures ...
... unit cell 23,-, y,, z,- the fractional coordinates of the jib atom in the unit cell h, k, l indices of a reflection; coordinates of a reciprocal lattice point. h is also used for the axial rise per unit in describing helical structures ...
Page 62
... averaging process renders the pattern more difficult to use for structural studies than is the case with macroscopically crystalline materials. Ill. The Protein Crystal A. THE UNIT CELL In a. 62 R. D. B. FRASER AND T. P. MACRAE.
... averaging process renders the pattern more difficult to use for structural studies than is the case with macroscopically crystalline materials. Ill. The Protein Crystal A. THE UNIT CELL In a. 62 R. D. B. FRASER AND T. P. MACRAE.
Page 63
... unit cell in three dimensions as shown in Fig. 2. The unit cell is characterized by the lengths a, b, and c of its edges and the angles 5s “'9' ~ til FIG. 2. A crystal may be regarded as being built up by the three-dimensional ...
... unit cell in three dimensions as shown in Fig. 2. The unit cell is characterized by the lengths a, b, and c of its edges and the angles 5s “'9' ~ til FIG. 2. A crystal may be regarded as being built up by the three-dimensional ...
Page 64
... unit cell are equal to the distances from the axes, measured parallel to the unit cell edges, expressed as fractions of the respective cell edges. In the case illustrated, the fractional coordinates of the atom are x I 0.75, y I 0.7, z ...
... unit cell are equal to the distances from the axes, measured parallel to the unit cell edges, expressed as fractions of the respective cell edges. In the case illustrated, the fractional coordinates of the atom are x I 0.75, y I 0.7, z ...
Contents
59 | |
Chapter 3 Ultraviolet Absorption | 101 |
Chapter 4 Fluorescence of Proteins | 171 |
Chapter 5 Perturbation and Flow Techniques | 245 |
Chapter 6 Dielectric Properties of Proteins I Dielectric Relaxation | 291 |
Chapter 7 Dielectric Properties of Proteins II Electric Birefringence and Dichroism | 335 |
Chapter 8 Electrophoresis | 369 |
Chapter 9 Analytical Gel Filtration | 451 |
Author Index | 497 |
Subject Index | 509 |
Common terms and phrases
absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient concentration curve defined denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction diffusion dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission energy enzyme equation equilibrium excitation experimental factor field strength film filters first flow fluorescence fraction frequency gel filtration groups intensity interactions ionic strength ions light macromolecules magnification measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic phenylalanine photomultiplier Phys plot polarization polymer protein quantum yield ratio reaction reflections relaxation residues ribonuclease rotation shown in Fig significant solution solvent specific specimen spectra structure sufficiently technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone