Applied and Environmental Microbiology, Volume 54, Pages 851-1642American Society for Microbiology, 1988 - Microbial ecology |
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Page 985
... buffer A , and the intracellular D - aminoacylase activity was deter- mined . Enzyme assay . The D - aminoacylase activity was measured by reacting TNBS with the D - methionine released from N - acetyl - D - methionine , which was shown ...
... buffer A , and the intracellular D - aminoacylase activity was deter- mined . Enzyme assay . The D - aminoacylase activity was measured by reacting TNBS with the D - methionine released from N - acetyl - D - methionine , which was shown ...
Page 1086
... buffer , and extensively dialyzed in TE buffer . DNA concentrations were estimated spectropho- tometrically at 260 nm . DNA purity was checked by scan- ning the absorbance of DNA samples between 200 and 400 nm and monitoring the ...
... buffer , and extensively dialyzed in TE buffer . DNA concentrations were estimated spectropho- tometrically at 260 nm . DNA purity was checked by scan- ning the absorbance of DNA samples between 200 and 400 nm and monitoring the ...
Page 1132
... buffer and subsequently with 5 ml of soni- cation buffer ( 50 mM potassium phosphate , pH 7 , 10 mM MgSO4 ) . Enzyme activities present in the supernatant after incubation of the cells in enzyme release buffer and in the supernatants ...
... buffer and subsequently with 5 ml of soni- cation buffer ( 50 mM potassium phosphate , pH 7 , 10 mM MgSO4 ) . Enzyme activities present in the supernatant after incubation of the cells in enzyme release buffer and in the supernatants ...
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acenaphthene acetate activity aerobic agar alghumin amino acids anaerobic analysis antibiotic Appl arginine aromatic assay ATCC bacteria Bacteriol bacteriophage bacterium Bacteroides Biol biomass broth buffer carbon carotovora cell wall cellulose chemical chromatography cloned coli colonies compounds concentration containing cremoris culture degradation denitrification detected determined digestion dilution effect Environ enzyme Escherichia coli ethanol fermentation ferulic acid filter fragment gene genetic glucose growth H₂ hybridization incubated infection inhibition inoculated inoculum isolated laboratory lactis lignin lividans medium metabolism metabolites methanogenic method methylcellulose microbial Microbiol microorganisms molecular mutant naphthalene nitrate obtained organic oxide phage phenanthrene phenol plasmid plates present production protein protoplast Pseudomonas resistance rhizobia Rhizobium rumen ruminal samples sediments Society for Microbiology soil solution species spores sterile strains Streptococcus Streptomyces substrate subtilis Table temperature tetracycline tion toxin transfer transformation xylanase yeast extract µg/ml