Applied and Environmental Microbiology, Volume 54, Pages 851-1642American Society for Microbiology, 1988 - Microbial ecology |
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Page 1132
... cell wall enzyme , and cell - bound en- zyme is described below . ( i ) Supernatant enzyme . Samples ( 100 ml ) of steady - state cultures ( containing approximately 0.1 g [ dry weight ] of cells ) were harvested by centrifugation at 4 ...
... cell wall enzyme , and cell - bound en- zyme is described below . ( i ) Supernatant enzyme . Samples ( 100 ml ) of steady - state cultures ( containing approximately 0.1 g [ dry weight ] of cells ) were harvested by centrifugation at 4 ...
Page 1133
... cells in a 50 mM potassium phosphate buffer , pH 7 , or in a 100 mM sodium acetate buffer , pH 4.5 , released 40 to 70 % and 30 to 40 % , respectively , of the cell wall enzyme within 1 h . These amounts increased during prolonged ...
... cells in a 50 mM potassium phosphate buffer , pH 7 , or in a 100 mM sodium acetate buffer , pH 4.5 , released 40 to 70 % and 30 to 40 % , respectively , of the cell wall enzyme within 1 h . These amounts increased during prolonged ...
Page 1134
... cell wall , and cell - bound fractions was dependent on the nature of the growth - limiting carbon substrate . Especially the cell - bound enzyme was present at very low levels when lactose or glucose was used as carbon source ( Table 2 ) ...
... cell wall , and cell - bound fractions was dependent on the nature of the growth - limiting carbon substrate . Especially the cell - bound enzyme was present at very low levels when lactose or glucose was used as carbon source ( Table 2 ) ...
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acenaphthene acetate activity aerobic agar alghumin amino acids anaerobic analysis antibiotic Appl arginine aromatic assay ATCC bacteria Bacteriol bacteriophage bacterium Bacteroides Biol biomass broth buffer carbon carotovora cell wall cellulose chemical chromatography cloned coli colonies compounds concentration containing cremoris culture degradation denitrification detected determined digestion dilution effect Environ enzyme Escherichia coli ethanol fermentation ferulic acid filter fragment gene genetic glucose growth H₂ hybridization incubated infection inhibition inoculated inoculum isolated laboratory lactis lignin lividans medium metabolism metabolites methanogenic method methylcellulose microbial Microbiol microorganisms molecular mutant naphthalene nitrate obtained organic oxide phage phenanthrene phenol plasmid plates present production protein protoplast Pseudomonas resistance rhizobia Rhizobium rumen ruminal samples sediments Society for Microbiology soil solution species spores sterile strains Streptococcus Streptomyces substrate subtilis Table temperature tetracycline tion toxin transfer transformation xylanase yeast extract µg/ml