Applied and Environmental Microbiology, Volume 54, Pages 851-1642American Society for Microbiology, 1988 - Microbial ecology |
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Page 985
... column chromatography . The active enzyme fraction from DEAE - Sephadex A - 50 column was applied to a Sephadex G - 100 column ( 6.0 by 130 cm ) . The column was eluted with buffer A at a flow rate of 60 ml / h . ( v ) Fractogel TSK ...
... column chromatography . The active enzyme fraction from DEAE - Sephadex A - 50 column was applied to a Sephadex G - 100 column ( 6.0 by 130 cm ) . The column was eluted with buffer A at a flow rate of 60 ml / h . ( v ) Fractogel TSK ...
Page 1489
... column was applied to a chromatofocusing column ( 1.0 by 30 cm ) which was packed with Polybuffer exchanger PBE94 . The column had been preequilibrated with 12 bed volumes ( 300 ml ) of 0.025 M piperazine hydro- chloride ( pH 5.5 ) ...
... column was applied to a chromatofocusing column ( 1.0 by 30 cm ) which was packed with Polybuffer exchanger PBE94 . The column had been preequilibrated with 12 bed volumes ( 300 ml ) of 0.025 M piperazine hydro- chloride ( pH 5.5 ) ...
Page 1524
... column chromatography of fraction A from Sephadex G - 50 . Symbols : A280 ; △ , cellobiohydrolase activity , with Avicel as the substrate ; ○ , endoglucanase activity ; — , phosphate concentration . Experimental details are described ...
... column chromatography of fraction A from Sephadex G - 50 . Symbols : A280 ; △ , cellobiohydrolase activity , with Avicel as the substrate ; ○ , endoglucanase activity ; — , phosphate concentration . Experimental details are described ...
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acenaphthene acetate activity aerobic agar alghumin amino acids anaerobic analysis antibiotic Appl arginine aromatic assay ATCC bacteria Bacteriol bacteriophage bacterium Bacteroides Biol biomass broth buffer carbon carotovora cell wall cellulose chemical chromatography cloned coli colonies compounds concentration containing cremoris culture degradation denitrification detected determined digestion dilution effect Environ enzyme Escherichia coli ethanol fermentation ferulic acid filter fragment gene genetic glucose growth H₂ hybridization incubated infection inhibition inoculated inoculum isolated laboratory lactis lignin lividans medium metabolism metabolites methanogenic method methylcellulose microbial Microbiol microorganisms molecular mutant naphthalene nitrate obtained organic oxide phage phenanthrene phenol plasmid plates present production protein protoplast Pseudomonas resistance rhizobia Rhizobium rumen ruminal samples sediments Society for Microbiology soil solution species spores sterile strains Streptococcus Streptomyces substrate subtilis Table temperature tetracycline tion toxin transfer transformation xylanase yeast extract µg/ml