Applied and Environmental Microbiology, Volume 54, Pages 851-1642American Society for Microbiology, 1988 - Microbial ecology |
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Page 867
... cremoris strains were performed by modifications of the method of McKay et al . ( 15 ) as described before ( 26 ) . RESULTS Protoplast formation , regeneration , and transformation . The repeated failure to obtain transformed S. cremoris ...
... cremoris strains were performed by modifications of the method of McKay et al . ( 15 ) as described before ( 26 ) . RESULTS Protoplast formation , regeneration , and transformation . The repeated failure to obtain transformed S. cremoris ...
Page 869
... cremoris Wg2L ( pGKV110 ) ( lanes 2 ) , S. cremoris Wg2L - 1 ( pGKV110 ) ( lanes 3 ) , S. cremoris Wg2L ( lanes 4 ) , S. cremoris E8L ( lanes 5 ) , S. cremoris E8L ( pGKV110 ) ( lanes 6 ) , S. cremoris HpL ( lanes 7 ) , S. cremoris HpL ...
... cremoris Wg2L ( pGKV110 ) ( lanes 2 ) , S. cremoris Wg2L - 1 ( pGKV110 ) ( lanes 3 ) , S. cremoris Wg2L ( lanes 4 ) , S. cremoris E8L ( lanes 5 ) , S. cremoris E8L ( pGKV110 ) ( lanes 6 ) , S. cremoris HpL ( lanes 7 ) , S. cremoris HpL ...
Page 870
... cremoris E8L and F16 , lacking plasmids which hybridized to pGKV110 ( Fig . 3 , lanes 5 , 6 , 9 , and 10 ) , were highly transformable with this plasmid . The only exception was S. cremoris H61 , which was poorly transformable despite ...
... cremoris E8L and F16 , lacking plasmids which hybridized to pGKV110 ( Fig . 3 , lanes 5 , 6 , 9 , and 10 ) , were highly transformable with this plasmid . The only exception was S. cremoris H61 , which was poorly transformable despite ...
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acenaphthene acetate activity aerobic agar alghumin amino acids anaerobic analysis antibiotic Appl arginine aromatic assay ATCC bacteria Bacteriol bacteriophage bacterium Bacteroides Biol biomass broth buffer carbon carotovora cell wall cellulose chemical chromatography cloned coli colonies compounds concentration containing cremoris culture degradation denitrification detected determined digestion dilution effect Environ enzyme Escherichia coli ethanol fermentation ferulic acid filter fragment gene genetic glucose growth H₂ hybridization incubated infection inhibition inoculated inoculum isolated laboratory lactis lignin lividans medium metabolism metabolites methanogenic method methylcellulose microbial Microbiol microorganisms molecular mutant naphthalene nitrate obtained organic oxide phage phenanthrene phenol plasmid plates present production protein protoplast Pseudomonas resistance rhizobia Rhizobium rumen ruminal samples sediments Society for Microbiology soil solution species spores sterile strains Streptococcus Streptomyces substrate subtilis Table temperature tetracycline tion toxin transfer transformation xylanase yeast extract µg/ml