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acetate acid activity added addition agar amount anaerobic analysis antibiotic appeared Appl assay bacteria bacteriophage buffer carbon cells cellulose chemical chromatography coli colonies compared compounds concentration containing culture degradation described detected determined digestion dilution effect Environ enzyme ethanol experiments extract fermentation filter formed fraction fragment gene glucose grown growth hybridization increase incubated indicated infection inhibition initial inoculated isolated laboratory levels material measured medium methanogenic method microbial Microbiol Microbiology mixed mutant natural nitrate observed obtained occurred organic oxide period plant plasmid plates prepared present previously production protein range ratio reaction reduction relative removed reported resistance respectively samples sediments selected showed shown similar soil solution species spores standard strains substrate suggest Table temperature tion transfer transformation treatment University values various yeast