Applied and Environmental Microbiology, Volume 54, Pages 851-1642American Society for Microbiology, 1988 - Microbial ecology |
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Page 865
Several such systems have recently been described , such as transductional and
conjugal transfer of plasmids in S. lactis , S. lactis subsp . diacetylactis , and S.
cremoris , transformation of protoplasts by plasmid DNA , protoplast fusions ...
Several such systems have recently been described , such as transductional and
conjugal transfer of plasmids in S. lactis , S. lactis subsp . diacetylactis , and S.
cremoris , transformation of protoplasts by plasmid DNA , protoplast fusions ...
Page 867
Transformation of various S. cremoris strains on different regeneration media
Transformation efficiency " with plasmid : Strain PGKV110 ... Colonies of
transformed cells became visible after 48 h of incubation at 30 ° C . Blot
hybridization .
Transformation of various S. cremoris strains on different regeneration media
Transformation efficiency " with plasmid : Strain PGKV110 ... Colonies of
transformed cells became visible after 48 h of incubation at 30 ° C . Blot
hybridization .
Page 1306
Results of transformation and nutritional assays Intensity of transformation with
auxotroph " : No. of Growth on strains medium tested MB Interpretation MB4
MB21 + 4+ 4+ 4+ 4+ 19 2 1+ to 2+ + 1+ to 2+ 1+ to 2+ 1+ or - 1+ M. bovis
Auxotrophic ...
Results of transformation and nutritional assays Intensity of transformation with
auxotroph " : No. of Growth on strains medium tested MB Interpretation MB4
MB21 + 4+ 4+ 4+ 4+ 19 2 1+ to 2+ + 1+ to 2+ 1+ to 2+ 1+ or - 1+ M. bovis
Auxotrophic ...
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acetate acid activity added addition agar amount anaerobic analysis antibiotic appeared Appl assay bacteria bacteriophage buffer carbon cells cellulose chemical chromatography coli colonies compared compounds concentration containing culture degradation described detected determined digestion dilution effect Environ enzyme ethanol experiments extract fermentation filter formed fraction fragment gene glucose grown growth hybridization increase incubated indicated infection inhibition initial inoculated isolated laboratory levels material measured medium methanogenic method microbial Microbiol Microbiology mixed mutant natural nitrate observed obtained occurred organic oxide period phase plant plasmid plates prepared present previously production protein range ratio reaction reduction relative removed reported resistance respectively samples sediments selected showed shown similar soil solution species spores standard strains substrate suggest Table temperature tion transfer transformation treatment University values various yeast