Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 91
Page 2-18
... BamHI 33500 Xhol 33240 Sall 32750 Sall 31750 EcoRI 31620 Smal 27980 BamHI 27480 Hindill 26250 Pvul 26100 EcoRI 25160 Hindill 24760 Sacl 24510 Xbal 23130 Hindill 22430 Bg 22350 BamHI 21230 EcoRI 19400 Smal 18560 Kpn 17050 Koni 11930 Pvul ...
... BamHI 33500 Xhol 33240 Sall 32750 Sall 31750 EcoRI 31620 Smal 27980 BamHI 27480 Hindill 26250 Pvul 26100 EcoRI 25160 Hindill 24760 Sacl 24510 Xbal 23130 Hindill 22430 Bg 22350 BamHI 21230 EcoRI 19400 Smal 18560 Kpn 17050 Koni 11930 Pvul ...
Page 2-28
... BamHI 11930 Pvul 48500 Rend 44870 EcoRI 44140 Hindill 41730 BamHI 30090 Smal 39170 EcoR ! 38810 Bgl 38750 Bghi 38100 Boll 37460 Hindi 36900 Hindill 35790 Pvul 35710 Bgl 34500 BamHI 33500 Xhol 33240 San 32750 Sall 31750 EcoRI 31620 Smal ...
... BamHI 11930 Pvul 48500 Rend 44870 EcoRI 44140 Hindill 41730 BamHI 30090 Smal 39170 EcoR ! 38810 Bgl 38750 Bghi 38100 Boll 37460 Hindi 36900 Hindill 35790 Pvul 35710 Bgl 34500 BamHI 33500 Xhol 33240 San 32750 Sall 31750 EcoRI 31620 Smal ...
Page 2-40
... BamHI 21230 EcoRI 19400 Smal 18560 Kpnl 17050 Kpnl 11930 Pvul 6510 BamHI 43340 Rend 39000 Hindill 36590 BamHI 34740 Smal 33670 Bgl 33610 BglI 32470 Hindill 32710 EcoRI 30500 BamHI 29500 Xhol 29240 San 28750 Sall 27620 Smal 23970 BamHI ...
... BamHI 21230 EcoRI 19400 Smal 18560 Kpnl 17050 Kpnl 11930 Pvul 6510 BamHI 43340 Rend 39000 Hindill 36590 BamHI 34740 Smal 33670 Bgl 33610 BglI 32470 Hindill 32710 EcoRI 30500 BamHI 29500 Xhol 29240 San 28750 Sall 27620 Smal 23970 BamHI ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield