Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 5-30
... Activity greater than the activity observed in buffer recommended by the manufacturer ; ( +++ ) activity approximately equal to the activity observed in buffer recommended by the manufacturer ; ( ++ ) activity approximately 50-80 % of ...
... Activity greater than the activity observed in buffer recommended by the manufacturer ; ( +++ ) activity approximately equal to the activity observed in buffer recommended by the manufacturer ; ( ++ ) activity approximately 50-80 % of ...
Page 5-36
... activity . The RNAase H activity is essential for cell viability in E. coli but has not been used in molecular cloning . 1. Labeling of DNA by nick translation ( see Figure 5.2 ) . Of all the polymer- ases , only E. coli DNA polymerase ...
... activity . The RNAase H activity is essential for cell viability in E. coli but has not been used in molecular cloning . 1. Labeling of DNA by nick translation ( see Figure 5.2 ) . Of all the polymer- ases , only E. coli DNA polymerase ...
Page 5-39
... activity on double - stranded DNA is blocked by 5 ' - 3 ' DNA polymerase activity and is inhibited by dNMPs with 5 ' phosphates . Reaction : E. coli DNA polymerase I 5 ' NOH double- or single - stranded DNA Mg ++ For example : T3 ' 5 ...
... activity on double - stranded DNA is blocked by 5 ' - 3 ' DNA polymerase activity and is inhibited by dNMPs with 5 ' phosphates . Reaction : E. coli DNA polymerase I 5 ' NOH double- or single - stranded DNA Mg ++ For example : T3 ' 5 ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield