Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-90
... colonies carrying cosmids or plasmids that contain DNA sequences of interest . Methods are presented below to screen bacterial colonies with radiolabeled probes that are longer than 100 nucleotides in length . The preparation of these ...
... colonies carrying cosmids or plasmids that contain DNA sequences of interest . Methods are presented below to screen bacterial colonies with radiolabeled probes that are longer than 100 nucleotides in length . The preparation of these ...
Page 1-92
... Colonies to Nitrocellulose Filters This procedure is used when it is necessary to screen a small number of bacterial colonies ( 100-200 ) that are dispersed over several agar plates . The colonies are consolidated on a master agar plate ...
... Colonies to Nitrocellulose Filters This procedure is used when it is necessary to screen a small number of bacterial colonies ( 100-200 ) that are dispersed over several agar plates . The colonies are consolidated on a master agar plate ...
Page 1-93
... colonies simultaneously from the surfaces of agar plates to nitrocellulose filters . This method works with bacterial colonies of any size , but small colonies ( 0.1-0.2 mm ) give the best results ; they produce sharper hybridization ...
... colonies simultaneously from the surfaces of agar plates to nitrocellulose filters . This method works with bacterial colonies of any size , but small colonies ( 0.1-0.2 mm ) give the best results ; they produce sharper hybridization ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield