Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 56
Page 1-105
... Construction and characteriza- tion of new cloning vehicles . II . A mul- tipurpose cloning system . Gene 2:95 . Burns , D.M. and I.R. Beacham . 1984 . Positive selection vectors : A small plas- mid vector useful for the direct selec ...
... Construction and characteriza- tion of new cloning vehicles . II . A mul- tipurpose cloning system . Gene 2:95 . Burns , D.M. and I.R. Beacham . 1984 . Positive selection vectors : A small plas- mid vector useful for the direct selec ...
Page 1-110
... Construction of plasmids containing the coding sequences . Science 196 : 1313 . Vernet , T. , P.C.K. Lau , S.A. Narang , and L.P. Visentin . 1985. A direct - selection vector derived from pColE3 - CA38 and adapted for foreign gene ...
... Construction of plasmids containing the coding sequences . Science 196 : 1313 . Vernet , T. , P.C.K. Lau , S.A. Narang , and L.P. Visentin . 1985. A direct - selection vector derived from pColE3 - CA38 and adapted for foreign gene ...
Page 3-27
... construction of large cosmid libraries when limited amounts of genomic DNA are available . CLONING IN PHOSPHATASE - TREATED COSMID VECTORS The basic steps Cosmid Vectors 3.27 Construction of Genomic DNA Libraries in Cosmid Vectors 3 27.
... construction of large cosmid libraries when limited amounts of genomic DNA are available . CLONING IN PHOSPHATASE - TREATED COSMID VECTORS The basic steps Cosmid Vectors 3.27 Construction of Genomic DNA Libraries in Cosmid Vectors 3 27.
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield