Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 3-5
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Cloning in Cosmid Vectors In their simplest form , cosmid vectors are modified plasmids that carry a copy of the DNA sequences ( cos sequences ) required for packaging DNA ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Cloning in Cosmid Vectors In their simplest form , cosmid vectors are modified plasmids that carry a copy of the DNA sequences ( cos sequences ) required for packaging DNA ...
Page 3-18
... cosmids that carry selectable markers for eukaryotic cells are de- scribed on the following pages . pHC79-2cos / tk + pHC79-2cos / tk ( Lindenmaier et 3.18 Cosmid Vectors COSMID VECTORS FOR TRANSFECTION OF MAMMALIAN CELLS 3 18.
... cosmids that carry selectable markers for eukaryotic cells are de- scribed on the following pages . pHC79-2cos / tk + pHC79-2cos / tk ( Lindenmaier et 3.18 Cosmid Vectors COSMID VECTORS FOR TRANSFECTION OF MAMMALIAN CELLS 3 18.
Page 3-52
... Cosmids Because circular cosmid molecules carried in bacterial cells contain a cos site , they can be efficiently packaged into bacteriophage A particles . When cosmid - containing bacteria are infected with bacteriophage ( or when a ...
... Cosmids Because circular cosmid molecules carried in bacterial cells contain a cos site , they can be efficiently packaged into bacteriophage A particles . When cosmid - containing bacteria are infected with bacteriophage ( or when a ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield