Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 47
Page 3-19
... derived from SV2 - gpt ( Mulligan and Berg 1980 ) . pCV107 contains the mouse dihydrofolate reductase ( dhfr ) gene and the SV40 origin of replication , both derived from SV2 - dhfr ( Subramani et al . 1981 ) . pCV108 contains the ...
... derived from SV2 - gpt ( Mulligan and Berg 1980 ) . pCV107 contains the mouse dihydrofolate reductase ( dhfr ) gene and the SV40 origin of replication , both derived from SV2 - dhfr ( Subramani et al . 1981 ) . pCV108 contains the ...
Page 4-44
... ( derived from M13mp1 ) , a plasmid origin of replication ( derived from p15A [ Selzer et al . 1983 ] ) , and the kanamycin resistance gene from Tn903 ( Grindley and Joyce 1980 ) at the Aval site ( nucleotide 5825 of bacteriophage M13 ) ...
... ( derived from M13mp1 ) , a plasmid origin of replication ( derived from p15A [ Selzer et al . 1983 ] ) , and the kanamycin resistance gene from Tn903 ( Grindley and Joyce 1980 ) at the Aval site ( nucleotide 5825 of bacteriophage M13 ) ...
Page 4-50
... ( derived from JM83 ; see Table 4.1 ) and MV1190 ( derived from JM101 ) produce satisfactory yields , whereas MV1304 ( derived from JM105 ) does not . JM109 carrying pUC118 or pUC119 or plasmids derived from them undergoes significant ...
... ( derived from JM83 ; see Table 4.1 ) and MV1190 ( derived from JM101 ) produce satisfactory yields , whereas MV1304 ( derived from JM105 ) does not . JM109 carrying pUC118 or pUC119 or plasmids derived from them undergoes significant ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield