Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 4-41
... described in Chapter 2 , pages 2.109-2.111 . Note The bacteriophage M13 DNA transferred to the filter does not need to be denatured with alkali . Instead , after removing the filter from the surface of the agar or agarose , allow it to ...
... described in Chapter 2 , pages 2.109-2.111 . Note The bacteriophage M13 DNA transferred to the filter does not need to be denatured with alkali . Instead , after removing the filter from the surface of the agar or agarose , allow it to ...
Page 6-13
... described on page 6.15 . The presence of ethidium bromide allows the gel to be examined by ultraviolet illumination at any stage during electrophoresis . However , some people feel that sharper bands of DNA are obtained when the gel is ...
... described on page 6.15 . The presence of ethidium bromide allows the gel to be examined by ultraviolet illumination at any stage during electrophoresis . However , some people feel that sharper bands of DNA are obtained when the gel is ...
Page 6-46
... described by Maxam and Gilbert ( 1977 ) . The DNA obtained is of very high purity and is free of contaminants that ... described on pages 6.24-6.27 . " Crush and Soak " Method The following procedure is a modification of the technique ...
... described by Maxam and Gilbert ( 1977 ) . The DNA obtained is of very high purity and is free of contaminants that ... described on pages 6.24-6.27 . " Crush and Soak " Method The following procedure is a modification of the technique ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield