Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-72
... desired restriction fragment of foreign DNA and the appropriate segment of plasmid DNA . In the protocol given below ( adapted from Struhl 1985 by S. Michaelis , pers . comm . ) , ligation of plasmid and foreign DNAs is carried out ...
... desired restriction fragment of foreign DNA and the appropriate segment of plasmid DNA . In the protocol given below ( adapted from Struhl 1985 by S. Michaelis , pers . comm . ) , ligation of plasmid and foreign DNAs is carried out ...
Page 2-82
... desired se- quences of foreign DNA . 5. Plaque purification of selected recombinant bacteriophages , followed by analysis of the foreign DNA sequences . PREPARATION OF VECTOR DNA In some cases , bacteriophage λ DNA can be prepared for ...
... desired se- quences of foreign DNA . 5. Plaque purification of selected recombinant bacteriophages , followed by analysis of the foreign DNA sequences . PREPARATION OF VECTOR DNA In some cases , bacteriophage λ DNA can be prepared for ...
Page 5-73
... desired sequence next to a promoter or other controlling element , or to attach synthetic linkers at desired sites in the DNA . 2. Mapping restriction sites in DNA ( Legerski et al . 1978 ) . 3. Mapping secondary structure in DNA , for ...
... desired sequence next to a promoter or other controlling element , or to attach synthetic linkers at desired sites in the DNA . 2. Mapping restriction sites in DNA ( Legerski et al . 1978 ) . 3. Mapping secondary structure in DNA , for ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield