Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-83
... digestion products are present . 5. If digestion is incomplete , warm the reaction to the appropriate tempera- ture , add more restriction enzyme ( 50-100 units ) , and continue the incubation at the optimal temperature recommended by ...
... digestion products are present . 5. If digestion is incomplete , warm the reaction to the appropriate tempera- ture , add more restriction enzyme ( 50-100 units ) , and continue the incubation at the optimal temperature recommended by ...
Page 2-92
... Digestion of such a vector with both BamHI and EcoRI yields left and right arms that carry BamHI termini , a stuffer fragment carrying EcoRI termini , and short segments of the polycloning sites carrying EcoRI and BamHI termini . These ...
... Digestion of such a vector with both BamHI and EcoRI yields left and right arms that carry BamHI termini , a stuffer fragment carrying EcoRI termini , and short segments of the polycloning sites carrying EcoRI and BamHI termini . These ...
Page 1-17
... digestion with restriction en- zymes , 3.32 , 9.24-9.29 partial filling of recessed 3 ' termini of fragments , 9.29-9.30 pulsed - field gel electrophoresis , 6.50- 6.52 DNA preparation , 6.53-6.57 Southern hybridization analysis . See ...
... digestion with restriction en- zymes , 3.32 , 9.24-9.29 partial filling of recessed 3 ' termini of fragments , 9.29-9.30 pulsed - field gel electrophoresis , 6.50- 6.52 DNA preparation , 6.53-6.57 Southern hybridization analysis . See ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield