Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 73
Page 7-8
... ethanol . Stand the open tube on the bench for a few minutes to allow the last traces of ethanol to evaporate . 13. Redissolve the pellet in 200 μl of TE ( pH 7.6 ) . Add 500 μl of ethanol , and store the preparation at -70 ° C until it ...
... ethanol . Stand the open tube on the bench for a few minutes to allow the last traces of ethanol to evaporate . 13. Redissolve the pellet in 200 μl of TE ( pH 7.6 ) . Add 500 μl of ethanol , and store the preparation at -70 ° C until it ...
Page 7-21
... ethanol at room temperature . Invert the tube and drain off the ethanol , again checking that the pellet of RNA is not dislodged . Washing with ethanol removes CsCl from the pellet of RNA , making it easier to dissolve . 9. Allow the ...
... ethanol at room temperature . Invert the tube and drain off the ethanol , again checking that the pellet of RNA is not dislodged . Washing with ethanol removes CsCl from the pellet of RNA , making it easier to dissolve . 9. Allow the ...
Page 7-62
... ethanol . Store the solution at 0 ° C for at least 30 minutes . Recover the nucleic acids by centrifuga- tion at 12,000g for 15 minutes at 4 ° C in a microfuge . Discard the supernatant , wash the pellet with 70 % ethanol , and ...
... ethanol . Store the solution at 0 ° C for at least 30 minutes . Recover the nucleic acids by centrifuga- tion at 12,000g for 15 minutes at 4 ° C in a microfuge . Discard the supernatant , wash the pellet with 70 % ethanol , and ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield