Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xxxii
... Eukaryotic Replicon 16.17 Plasmid DNA Expression Vectors Containing Regulatory Elements from Eukaryotic Viruses 16.17 SIMIAN VIRUS 40 VECTORS 16.17 BOVINE PAPILLOMAVIRUS VECTORS 16.23 EPSTEIN - BARR VIRUS VECTORS Amplification Systems ...
... Eukaryotic Replicon 16.17 Plasmid DNA Expression Vectors Containing Regulatory Elements from Eukaryotic Viruses 16.17 SIMIAN VIRUS 40 VECTORS 16.17 BOVINE PAPILLOMAVIRUS VECTORS 16.23 EPSTEIN - BARR VIRUS VECTORS Amplification Systems ...
Page 3-33
... eukaryotic DNA . Because of the inability of the vector to self - ligate , the reaction can be driven with a molar excess of vector molecules . The following ligation reactions contain a 9 : 1 molar ratio of vector DNA : eukaryotic DNA ...
... eukaryotic DNA . Because of the inability of the vector to self - ligate , the reaction can be driven with a molar excess of vector molecules . The following ligation reactions contain a 9 : 1 molar ratio of vector DNA : eukaryotic DNA ...
Page 3-40
... eukaryotic DNA . 1. Set up two reaction mixtures as follows : Reaction a fragments of eukaryotic DNA ( 35-45 kb in size ) HindIII / BamHI fragment of pJB8 2 με 1 με Sall / BamHI fragment of pJB8 1 με 10 ligation buffer ( see page 3.29 ...
... eukaryotic DNA . 1. Set up two reaction mixtures as follows : Reaction a fragments of eukaryotic DNA ( 35-45 kb in size ) HindIII / BamHI fragment of pJB8 2 με 1 με Sall / BamHI fragment of pJB8 1 με 10 ligation buffer ( see page 3.29 ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield