Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-95
... extracts prepared from bacteria infected with bacteriophage A mutant in genes required for assembly of bacteriophage ... extracts have been prepared from cells infected with A and E or D and E strains ; alternatively , extracts prepared ...
... extracts prepared from bacteria infected with bacteriophage A mutant in genes required for assembly of bacteriophage ... extracts have been prepared from cells infected with A and E or D and E strains ; alternatively , extracts prepared ...
Page 2-98
... extracts . The methods given below will be of use primarily to workers who use packaging extracts in such large quantity that it would be improvident to purchase them . Packaging extracts are usually prepared by growing the appropriate ...
... extracts . The methods given below will be of use primarily to workers who use packaging extracts in such large quantity that it would be improvident to purchase them . Packaging extracts are usually prepared by growing the appropriate ...
Page 2-104
... extract . 2. Mix gently . When the combined extracts are almost totally thawed , add the DNA to be packaged ( up to 1 μg dissolved in 5 μl of 10 mM Tris - Cl [ pH 8.0 ] , 10 mM MgCl2 ) . Mix with a fine glass stirring rod ( e.g. , a ...
... extract . 2. Mix gently . When the combined extracts are almost totally thawed , add the DNA to be packaged ( up to 1 μg dissolved in 5 μl of 10 mM Tris - Cl [ pH 8.0 ] , 10 mM MgCl2 ) . Mix with a fine glass stirring rod ( e.g. , a ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield