Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 84
Page 1-54
... inserted in only one orientation within recombinant plasmid restriction sites at junctions between plasmid and foreign DNAs are usually conserved foreign DNA can be inserted in either orientation recombinant plasmids may carry tandem ...
... inserted in only one orientation within recombinant plasmid restriction sites at junctions between plasmid and foreign DNAs are usually conserved foreign DNA can be inserted in either orientation recombinant plasmids may carry tandem ...
Page 1-87
... insertion of a segment of rat pre- proinsulin cDNA into the PstI site of pBR322 did not inactivate the amp gene . Presumably , a small piece of foreign DNA had been inserted that did not alter the reading frame of the amp ' gene , so ...
... insertion of a segment of rat pre- proinsulin cDNA into the PstI site of pBR322 did not inactivate the amp gene . Presumably , a small piece of foreign DNA had been inserted that did not alter the reading frame of the amp ' gene , so ...
Page 4-7
... inserted . This noncoding region is not dispensable , since it contains a cis - acting signal for the packaging and orientation of the DNA within bacteriophage particles ( Dotto et al . 1981 ; Webster et al . 1981 ) , sites for the ...
... inserted . This noncoding region is not dispensable , since it contains a cis - acting signal for the packaging and orientation of the DNA within bacteriophage particles ( Dotto et al . 1981 ; Webster et al . 1981 ) , sites for the ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield