Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-68
... ligase buffer bacteriophage T4 DNA ligase 5 mм ATP Incubate the reactions for 1-4 hours at 16 ° C . 10 ... ligation reactions 1.68 Plasmid Vectors Ligation of Cohesive Termini 1.
... ligase buffer bacteriophage T4 DNA ligase 5 mм ATP Incubate the reactions for 1-4 hours at 16 ° C . 10 ... ligation reactions 1.68 Plasmid Vectors Ligation of Cohesive Termini 1.
Page 1-70
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Ligation of Blunt - ended DNA Bacteriophage T4 DNA ligase , unlike E. coli DNA ligase , can catalyze the ligation of blunt - ended fragments of DNA ( Sgaramella and ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Ligation of Blunt - ended DNA Bacteriophage T4 DNA ligase , unlike E. coli DNA ligase , can catalyze the ligation of blunt - ended fragments of DNA ( Sgaramella and ...
Page 5-62
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Bacteriophage T4 DNA Ligase ( Bacteriophage T4 - infected E. coli ) USES r Bacteriophage T4 DNA ligase , a polypeptide of M , = 68,000 , catalyzes the formation of ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Bacteriophage T4 DNA Ligase ( Bacteriophage T4 - infected E. coli ) USES r Bacteriophage T4 DNA ligase , a polypeptide of M , = 68,000 , catalyzes the formation of ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield