Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 45
Page 1-23
... linear and closed circular DNA molecules . Ethidium bromide binds to DNA by intercalating between the bases , causing the double helix to unwind . This leads to an increase in the length of linear DNA molecules and to the introduction ...
... linear and closed circular DNA molecules . Ethidium bromide binds to DNA by intercalating between the bases , causing the double helix to unwind . This leads to an increase in the length of linear DNA molecules and to the introduction ...
Page 1-56
... linear molecules , adjust the salt concentration appropriately and add the second enzyme . At the same time , set up a separate pilot reaction that contains circular plasmid DNA and only the second of the two restriction enzymes . When ...
... linear molecules , adjust the salt concentration appropriately and add the second enzyme . At the same time , set up a separate pilot reaction that contains circular plasmid DNA and only the second of the two restriction enzymes . When ...
Page 2-4
... linear genomes COS COS cleavage by A protein COS encapsidation FIGURE 2.1 A schematic diagram of the bacteriophage A life cycle . Double - stranded bacteriophage λ DNA , which enters the bacterial host as a linear duplex , is indicated ...
... linear genomes COS COS cleavage by A protein COS encapsidation FIGURE 2.1 A schematic diagram of the bacteriophage A life cycle . Double - stranded bacteriophage λ DNA , which enters the bacterial host as a linear duplex , is indicated ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield