Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-94
... mixture . Optimum values for both of these parameters can be estimated from theoretical considerations ( see Chapter 1 , pages 1.63-1.67 ) . By necessity , however , such calculations assume that all the DNA molecules in the ligation ...
... mixture . Optimum values for both of these parameters can be estimated from theoretical considerations ( see Chapter 1 , pages 1.63-1.67 ) . By necessity , however , such calculations assume that all the DNA molecules in the ligation ...
Page 6-54
... mixture with a sealed pasteur pipette to ensure that the cells are evenly dispersed throughout the agarose . 4. Pipette the molten mixture into preformed Plexiglas molds ( 50-100 μl ) or draw the mixture into an appropriate length of ...
... mixture with a sealed pasteur pipette to ensure that the cells are evenly dispersed throughout the agarose . 4. Pipette the molten mixture into preformed Plexiglas molds ( 50-100 μl ) or draw the mixture into an appropriate length of ...
Page 7-75
... mixture to a water bath set at the annealing temperature . Incubate the mixture for 8-12 hours . The optimal temperature for annealing varies from RNA to RNA , presumably depending on such factors as G + C content and propensity to form ...
... mixture to a water bath set at the annealing temperature . Incubate the mixture for 8-12 hours . The optimal temperature for annealing varies from RNA to RNA , presumably depending on such factors as G + C content and propensity to form ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield