Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-98
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Preparation of Packaging Extracts and Packaging of Bacteriophage λ DNA In Vitro Packaging extracts are available at reasonable cost from commercial sources . These work ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Preparation of Packaging Extracts and Packaging of Bacteriophage λ DNA In Vitro Packaging extracts are available at reasonable cost from commercial sources . These work ...
Page 2-104
... packaging reactions are stored in closed tubes at 4 ° C . Notes i . Each batch of extracts should be tested for packaging efficiency with a standardized preparation of intact bacteriophage à DNA . ii . These extracts exhibit a high ...
... packaging reactions are stored in closed tubes at 4 ° C . Notes i . Each batch of extracts should be tested for packaging efficiency with a standardized preparation of intact bacteriophage à DNA . ii . These extracts exhibit a high ...
Page 3-33
... packaging extracts ( Bates and Swift 1983 ) . Extracts used to package cosmids should be prepared using a buffer that contains spermidine but not putrescine . When putrescine is omitted from the packaging extract and the packaging ...
... packaging extracts ( Bates and Swift 1983 ) . Extracts used to package cosmids should be prepared using a buffer that contains spermidine but not putrescine . When putrescine is omitted from the packaging extract and the packaging ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield