Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 62
Page 2-63
... plaques . For the same reason , it is advisable to pick plaques shortly after the bacterial lawn has grown up and the bacteriophage plaques have first appeared . Picking plaques early in their development also reduces the unwelcome ...
... plaques . For the same reason , it is advisable to pick plaques shortly after the bacterial lawn has grown up and the bacteriophage plaques have first appeared . Picking plaques early in their development also reduces the unwelcome ...
Page 2-108
... plaques by hybridization with 32P - labeled DNA probes ( Benton and Davis 1977 ) . Bacteriophages are plated at an appropriate density , and an imprint of the pattern of plaques is obtained by gently layering a nitrocellulose filter ...
... plaques by hybridization with 32P - labeled DNA probes ( Benton and Davis 1977 ) . Bacteriophages are plated at an appropriate density , and an imprint of the pattern of plaques is obtained by gently layering a nitrocellulose filter ...
Page 4-23
... plaques will be fully developed after 8-12 hours , and the blue color will intensify further if the plates are stored for a few hours at 4 ° C . Notes i . Colorless plaques ( formed by bacteriophages that are unable to accom- plish a ...
... plaques will be fully developed after 8-12 hours , and the blue color will intensify further if the plates are stored for a few hours at 4 ° C . Notes i . Colorless plaques ( formed by bacteriophages that are unable to accom- plish a ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
95 other sections not shown
Other editions - View all
Common terms and phrases
activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield