Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xii
... Plasmid DNA 1.31 Rapid Disruption of Bacterial Colonies to Test the Size of Plasmids 1.32 LARGE - SCALE PREPARATIONS OF PLASMID DNA 1.33 Amplification of Plasmids in Rich Medium 1.33 Harvesting and Lysis of Bacteria 1.34 HARVESTING 1.34 ...
... Plasmid DNA 1.31 Rapid Disruption of Bacterial Colonies to Test the Size of Plasmids 1.32 LARGE - SCALE PREPARATIONS OF PLASMID DNA 1.33 Amplification of Plasmids in Rich Medium 1.33 Harvesting and Lysis of Bacteria 1.34 HARVESTING 1.34 ...
Page 1-22
... plasmid , the strain of E. coli , and the technique that is to be used subsequently to purify the plasmid DNA . Although it is impractical to give precise conditions for each combination of plasmid and host , the following general ...
... plasmid , the strain of E. coli , and the technique that is to be used subsequently to purify the plasmid DNA . Although it is impractical to give precise conditions for each combination of plasmid and host , the following general ...
Page 1-56
... DNA by gel electrophoresis . If all of the plasmid DNA has been converted from circular to linear molecules , adjust the salt concentration appropriately and add the second enzyme . At the same time , set up a separate pilot reaction ...
... DNA by gel electrophoresis . If all of the plasmid DNA has been converted from circular to linear molecules , adjust the salt concentration appropriately and add the second enzyme . At the same time , set up a separate pilot reaction ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield