Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-76
... possible to obtain cultures of competent bacteria that yield 5 × 10 to 1 × 10 ° transformed colonies per microgram of supercoiled plasmid DNA . However , even the most experienced workers find it impossible to consistently generate ...
... possible to obtain cultures of competent bacteria that yield 5 × 10 to 1 × 10 ° transformed colonies per microgram of supercoiled plasmid DNA . However , even the most experienced workers find it impossible to consistently generate ...
Page 4-16
... possible time ( usually 4-8 hours , depending on the vector and the strain of E. coli ) and should not be used as a stock to seed further cultures . Recombinant bacteriophages carrying larger segments of foreign DNA almost always grow ...
... possible time ( usually 4-8 hours , depending on the vector and the strain of E. coli ) and should not be used as a stock to seed further cultures . Recombinant bacteriophages carrying larger segments of foreign DNA almost always grow ...
Page 5-73
... possible to distinguish fragments from the two termini and to deduce the order of the fragments in the unmapped DNA . BAL 31 can also be used to remove unwanted sequences from the termini of DNAs before cloning . After treatment with ...
... possible to distinguish fragments from the two termini and to deduce the order of the fragments in the unmapped DNA . BAL 31 can also be used to remove unwanted sequences from the termini of DNAs before cloning . After treatment with ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield