Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 88
Page 2-98
... prepared . Of the many protocols available for the preparation of packaging extracts , the two presented below are the simplest to perform and are highly efficient . Protocol I is representative of the classical type of packaging in ...
... prepared . Of the many protocols available for the preparation of packaging extracts , the two presented below are the simplest to perform and are highly efficient . Protocol I is representative of the classical type of packaging in ...
Page 2-100
... prepared from two different lysogenic strains . An extract containing preheads is prepared from strain BHB2690 by sonication , and an extract containing the D protein and other components necessary for packaging is prepared from strain ...
... prepared from two different lysogenic strains . An extract containing preheads is prepared from strain BHB2690 by sonication , and an extract containing the D protein and other components necessary for packaging is prepared from strain ...
Page 7-66
... prepared by phosphorylating the 5 ' terminus of the oligonucleotide primer ; uniformly labeled probes are prepared by incorporating radiolabeled dNTPs into the growing strand . In both cases , the newly synthesized strand of DNA can be ...
... prepared by phosphorylating the 5 ' terminus of the oligonucleotide primer ; uniformly labeled probes are prepared by incorporating radiolabeled dNTPs into the growing strand . In both cases , the newly synthesized strand of DNA can be ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield