Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-88
... presence of ampicillin or tetracycline . FIGURE 1.12 Insertional inactivation . Insertion of a segment of foreign DNA inactivates the tet ' gene . Bacteria transformed with this recombinant plasmid can grow in the presence of ampicillin ...
... presence of ampicillin or tetracycline . FIGURE 1.12 Insertional inactivation . Insertion of a segment of foreign DNA inactivates the tet ' gene . Bacteria transformed with this recombinant plasmid can grow in the presence of ampicillin ...
Page 5-56
... presence of a divalent cation , the enzyme catalyzes the addition of dNTPs to the 3 ' - hydroxyl termini of DNA molecules ( Bollum 1974 ) . When the nucleotide to be added is a purine , Mg ** is the preferred cation ; when the ...
... presence of a divalent cation , the enzyme catalyzes the addition of dNTPs to the 3 ' - hydroxyl termini of DNA molecules ( Bollum 1974 ) . When the nucleotide to be added is a purine , Mg ** is the preferred cation ; when the ...
Page 7-30
... PRESENCE OF METHYLMERCURIC HYDROXIDE Two methods are used to isolate mRNA molecules of particular size : elec- trophoresis through agarose gels and sedimentation through sucrose gradi- ents . Both methods have been used to estimate the ...
... PRESENCE OF METHYLMERCURIC HYDROXIDE Two methods are used to isolate mRNA molecules of particular size : elec- trophoresis through agarose gels and sedimentation through sucrose gradi- ents . Both methods have been used to estimate the ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield