Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 87
Page xxiv
... PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 Removal of ...
... PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 Removal of ...
Page xxv
... Probes 10.29 Preparation of DNA Template 10.31 Synthesis of RNA In Vitro 10.32 Synthesis of RNA Probes Radiolabeled to High Specific Activity 10.34 Troubleshooting 10.36 SYNTHESIS OF CDNA PROBES 10.38 Identification of cDNA Clones 10.38 ...
... Probes 10.29 Preparation of DNA Template 10.31 Synthesis of RNA In Vitro 10.32 Synthesis of RNA Probes Radiolabeled to High Specific Activity 10.34 Troubleshooting 10.36 SYNTHESIS OF CDNA PROBES 10.38 Identification of cDNA Clones 10.38 ...
Page 7-66
... Probes Single - stranded DNA probes are prepared by one of two methods : · Separation of the strands of a fragment of double - stranded DNA • De novo synthesis of a strand from a single - stranded template Strand - separated probes are ...
... Probes Single - stranded DNA probes are prepared by one of two methods : · Separation of the strands of a fragment of double - stranded DNA • De novo synthesis of a strand from a single - stranded template Strand - separated probes are ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield