Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-5
... proteins involved in DNA replication ) and terminate at tR2 . The leftward transcript codes for the N protein , whose action is essential for the next phase of infection . Delayed Early Transcription The N protein , by neutralizing the ...
... proteins involved in DNA replication ) and terminate at tR2 . The leftward transcript codes for the N protein , whose action is essential for the next phase of infection . Delayed Early Transcription The N protein , by neutralizing the ...
Page 2-7
... protein and proteolytic processing of other components , depends on a protein ( the groE gene product ) supplied by the host . The resulting structures are called preheads . Packaging the bacteriophage genome into preheads involves two ...
... protein and proteolytic processing of other components , depends on a protein ( the groE gene product ) supplied by the host . The resulting structures are called preheads . Packaging the bacteriophage genome into preheads involves two ...
Page 5-87
... Protein ( SSB ) ( E. coli or bacteriophage T4 - infected E. coli ) USES SSBS bind cooperatively to single - stranded DNA , but not to double - stranded DNA . Their usefulness ... Proteins 5 87 Single-stranded DNA-binding Protein (SSB) 5 87.
... Protein ( SSB ) ( E. coli or bacteriophage T4 - infected E. coli ) USES SSBS bind cooperatively to single - stranded DNA , but not to double - stranded DNA . Their usefulness ... Proteins 5 87 Single-stranded DNA-binding Protein (SSB) 5 87.
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield