Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 65
Page 4-7
... region , located between genes II and IV , into which seg- ments of foreign DNA may be inserted . This noncoding region is not dispensable , since it contains a cis - acting signal for the packaging and orientation of the DNA within ...
... region , located between genes II and IV , into which seg- ments of foreign DNA may be inserted . This noncoding region is not dispensable , since it contains a cis - acting signal for the packaging and orientation of the DNA within ...
Page 4-17
... region of a filamentous bacteriophage . This region contains all of the sequences required in cis for initiation and termination of viral DNA synthesis ( Dotto et al . 1984 ) and for morphogenesis of bacteriophage particles ( Dotto and ...
... region of a filamentous bacteriophage . This region contains all of the sequences required in cis for initiation and termination of viral DNA synthesis ( Dotto et al . 1984 ) and for morphogenesis of bacteriophage particles ( Dotto and ...
Page 4-18
... region pUC118 / 119 M13 M13K07 carries a mutated ( derived from version of gene II that MV1184 Vieira and Messing ( 1987 ) pUC18 / 19 ) works less well on its own pBluescript fl intergenic region than on that cloned in pUC118 / 119 ...
... region pUC118 / 119 M13 M13K07 carries a mutated ( derived from version of gene II that MV1184 Vieira and Messing ( 1987 ) pUC18 / 19 ) works less well on its own pBluescript fl intergenic region than on that cloned in pUC118 / 119 ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
95 other sections not shown
Other editions - View all
Common terms and phrases
activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield