Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 70
Page 1-6
... resistance ( tet1 ) gene carried on plasmid pBR322 encodes a 399 - amino - acid , membrane - associated protein ( Backman and Boyer 1983 ) that prevents the antibiotic from entering the cell ( Franklin 1967 ) . Ampicillin binds to and ...
... resistance ( tet1 ) gene carried on plasmid pBR322 encodes a 399 - amino - acid , membrane - associated protein ( Backman and Boyer 1983 ) that prevents the antibiotic from entering the cell ( Franklin 1967 ) . Ampicillin binds to and ...
Page 1-81
... resistance marker encoded by the plasmid . To maximize the efficiency of transformation , the cells should be gently ... resistance to tetracycline is used as the selectable marker . However , if resistance to ampicillin is selected ...
... resistance marker encoded by the plasmid . To maximize the efficiency of transformation , the cells should be gently ... resistance to tetracycline is used as the selectable marker . However , if resistance to ampicillin is selected ...
Page 3-19
... resistance gene . pHSG274 pHSG274 ( Brady et al . 1984 ) contains a kanamycin resistance gene that functions in both prokaryotes and eukaryotes and a ColE1 origin of replica- tion . Like pJB8 , the cos cloning site in pHSG274 is flanked ...
... resistance gene . pHSG274 pHSG274 ( Brady et al . 1984 ) contains a kanamycin resistance gene that functions in both prokaryotes and eukaryotes and a ColE1 origin of replica- tion . Like pJB8 , the cos cloning site in pHSG274 is flanked ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield