Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 48
Page 1-22
... resulting spheroplasts are lysed by adding a detergent such as SDS . This method minimizes the physical forces that are required to liberate the plasmid from the pressurized interior of the bacterium . 2. More severe methods can be used ...
... resulting spheroplasts are lysed by adding a detergent such as SDS . This method minimizes the physical forces that are required to liberate the plasmid from the pressurized interior of the bacterium . 2. More severe methods can be used ...
Page 2-45
... resulting small - plaque phenotype was altered by passing the vector through five generations of growth and picking a large plaque . Thus , the resulting vector is Spi ̄ and probably chi * . A polycloning site with SalI , SacI , XbaI ...
... resulting small - plaque phenotype was altered by passing the vector through five generations of growth and picking a large plaque . Thus , the resulting vector is Spi ̄ and probably chi * . A polycloning site with SalI , SacI , XbaI ...
Page 3-44
... resulting in over- or underrepresentation of certain sequences in the cosmid library . 3. Transduce the packaged cosmids into bacteria , plate the bacteria onto selective plates , and scrape the resulting colonies into TB containing the ...
... resulting in over- or underrepresentation of certain sequences in the cosmid library . 3. Transduce the packaged cosmids into bacteria , plate the bacteria onto selective plates , and scrape the resulting colonies into TB containing the ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield