Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 85
Page 2-13
... sequences . The final genetic factor to consider in choosing a vector is the presence of a chi site . Wild - type bacteriophage A contains no chi site , but mutants carrying an octanucleotide chi sequence were detected as suppressors of ...
... sequences . The final genetic factor to consider in choosing a vector is the presence of a chi site . Wild - type bacteriophage A contains no chi site , but mutants carrying an octanucleotide chi sequence were detected as suppressors of ...
Page 4-19
... sequences . Their system uses a phagemid that contains an intergenic region derived from wild - type M13 DNA that is efficiently recognized by the gene II product of the infecting bacteriophage . The genome of the infecting ...
... sequences . Their system uses a phagemid that contains an intergenic region derived from wild - type M13 DNA that is efficiently recognized by the gene II product of the infecting bacteriophage . The genome of the infecting ...
Page 5-50
... sequencing ( Chapter 13 ) and to amplify specific sequences of DNA in vitro by the polymerase chain reaction ( Chapter 14 ) . This is accomplished using two oligonucleotide primers complementary to sequences that flank the region of ...
... sequencing ( Chapter 13 ) and to amplify specific sequences of DNA in vitro by the polymerase chain reaction ( Chapter 14 ) . This is accomplished using two oligonucleotide primers complementary to sequences that flank the region of ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
95 other sections not shown
Other editions - View all
Common terms and phrases
activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield