Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 79
Page 4-19
... single - stranded phagemid sequences . Their system uses a phagemid that contains an intergenic region derived from wild - type M13 DNA that is efficiently recognized by the gene II product of the infecting bacteriophage . The genome of ...
... single - stranded phagemid sequences . Their system uses a phagemid that contains an intergenic region derived from wild - type M13 DNA that is efficiently recognized by the gene II product of the infecting bacteriophage . The genome of ...
Page 4-44
... single - stranded copies of the phagemid genome . The intergenic region of the phagemid is oriented so that the progeny virus particles contain the same strand of the lac region as bac- teriophages of the mp series ( Figure 4.6 ) ...
... single - stranded copies of the phagemid genome . The intergenic region of the phagemid is oriented so that the progeny virus particles contain the same strand of the lac region as bac- teriophages of the mp series ( Figure 4.6 ) ...
Page 4-49
... single - stranded DNA from the cultures that have produced the highest yield of bacteriophage particles . Note In principle , it should be possible to produce bacteriophage particles that contain single - stranded copies of plasmid DNA ...
... single - stranded DNA from the cultures that have produced the highest yield of bacteriophage particles . Note In principle , it should be possible to produce bacteriophage particles that contain single - stranded copies of plasmid DNA ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
95 other sections not shown
Other editions - View all
Common terms and phrases
activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield