Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 4-19
... single - stranded genome is efficiently converted to a double- stranded form in the usual way . However , subsequent amplification of the helper DNA can be driven by the plasmid origin of replication , and the requirement for the gene ...
... single - stranded genome is efficiently converted to a double- stranded form in the usual way . However , subsequent amplification of the helper DNA can be driven by the plasmid origin of replication , and the requirement for the gene ...
Page 7-58
... DNA templates • To locate the 5 ′ and 3 ′ splice junctions in relation to sites of cleavage with restriction enzymes in cloned genes or double - stranded ... single - stranded . DNA that has not formed duplexes is hydrolyzed with nuclease S1 ...
... DNA templates • To locate the 5 ′ and 3 ′ splice junctions in relation to sites of cleavage with restriction enzymes in cloned genes or double - stranded ... single - stranded . DNA that has not formed duplexes is hydrolyzed with nuclease S1 ...
Page 7-66
... stranded DNA Probes Single - stranded DNA probes are prepared by one of two methods : · Separation of the strands of a fragment of double - stranded DNA • De novo synthesis of a strand from a single - stranded template Strand ...
... stranded DNA Probes Single - stranded DNA probes are prepared by one of two methods : · Separation of the strands of a fragment of double - stranded DNA • De novo synthesis of a strand from a single - stranded template Strand ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield