Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-64
... termini in the solution . For double- stranded DNA with self - complementary cohesive termini , i = 2 NM × 10 3 ends / ml where N is Avogadro's number and M is the molar concentration of the DNA . Theoretically , when j = i , the end of ...
... termini in the solution . For double- stranded DNA with self - complementary cohesive termini , i = 2 NM × 10 3 ends / ml where N is Avogadro's number and M is the molar concentration of the DNA . Theoretically , when j = i , the end of ...
Page 5-11
... termini created by the filling of recessed 3 ' termini or the removal of protruding 3 ′ and 5 ′ termini . Blunt - end ligation can also be used to attach synthetic DNA linkers ( containing one or more restriction enzyme cleavage sites ) ...
... termini created by the filling of recessed 3 ' termini or the removal of protruding 3 ′ and 5 ′ termini . Blunt - end ligation can also be used to attach synthetic DNA linkers ( containing one or more restriction enzyme cleavage sites ) ...
Page 5-13
... termini allows as many as 70 % of the combinations of termini created by different restriction enzymes to be joined . Two empirical approaches for determining whether any two protruding 5 ' termini can be ligated have been reported ...
... termini allows as many as 70 % of the combinations of termini created by different restriction enzymes to be joined . Two empirical approaches for determining whether any two protruding 5 ' termini can be ligated have been reported ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield