Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xxiv
... TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS 9.34 Transfer of DNA to Nitrocellulose Filters 9.38 CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 9.38 SIMULTANEOUS TRANSFER OF DNA FROM A SINGLE AGAROSE GEL TO TWO ...
... TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS 9.34 Transfer of DNA to Nitrocellulose Filters 9.38 CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 9.38 SIMULTANEOUS TRANSFER OF DNA FROM A SINGLE AGAROSE GEL TO TWO ...
Page 7-46
... transfer , or electroblotting ( see Chapter 9 , pages 9.34-9.37 , for a discussion of the relative merits of these techniques ) . Capillary elution is carried out as described below ; vacuum transfer and electroblotting should be ...
... transfer , or electroblotting ( see Chapter 9 , pages 9.34-9.37 , for a discussion of the relative merits of these techniques ) . Capillary elution is carried out as described below ; vacuum transfer and electroblotting should be ...
Page 7-49
... transfer RNA from gels to nylon membranes are similar to those used for transfer to nitrocellulose filters and include vacuum trans- fer , electroblotting , or capillary elution ( see Chapter 9 , pages 9.34-9.37 , for a discussion of ...
... transfer RNA from gels to nylon membranes are similar to those used for transfer to nitrocellulose filters and include vacuum trans- fer , electroblotting , or capillary elution ( see Chapter 9 , pages 9.34-9.37 , for a discussion of ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
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activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield