Molecular Cloning: A Laboratory Manual, Volume 1The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. |
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... Electroporation works well with most commonly used laboratory strains of E. coli ( Dower et al . 1988 ; Tung and Chow 1995 ) . Unlike chemical transformation , the number of transformants generated by electropora- tion is marker ...
... ELECTROPORATION ) . Alternatively , the cell suspension may be snap - frozen and stored at -70 ° C for up to 6 months before electroporation , without loss of transforming efficiency . Because E. coli cells are small , they require very ...
... electroporation : • Length of pulse is determined mainly by the value of the capacitor and the conductivity of the medium . Most commercial electroporation machines use capacitative discharge to produce controlled pulses . Field ...