Molecular Cloning: A Laboratory Manual, Volume 1The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. |
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... genomic DNA should be at least eight times the capacity of the vector . This size range ensures that the majority of DNA molecules created by partial diges- tion with restriction enzyme ( s ) are derived from internal segments of the ...
... DNA for Use in Genomic Libraries : Preparative Reactions HIGH - MOLECULAR - WEIGHT DNA CAN BE FRAGMENTED by partial digestion with restriction enzymes . ( In particular circumstances , it may be desirable to perform a complete digestion ...
... DNA IS A REMARKABLY INERT CHEMICAL . Its potentially reactive groups are buried within the central helix , tied up in hydrogen bonds . Its base pairs ... genomic DNA . Protocol 1 Isolation of High - molecular - weight DNA Introduction 6.3.