Ancient DNA: Recovery and Analysis of Genetic Material from Paleontological, Archaeological, Museum, Medical, and Forensic SpecimensBernd Herrmann, Susanne Hummel Ancient DNA refers to DNA which can be recovered and analyzed from clinical, museum, archaeological and paleontological specimens. Ancient DNA ranges in age from less than 100 years to tens of millions of years. The study of ancient DNA is a young field, but it has been revolutionized by the application of polymerase chain reaction technology, and interest is growing very rapidly. Fields as diverse as evolution, anthropology, medicine, agriculture, and even law enforcement have quickly found applications in the recovery of ancient DNA. This book contains contributions from many of the "first generation" researchers who pioneered the development and application of ancient DNA methods. Their chapters present the protocols and precautions which have resulted in the remarkable results obtained in recent years. The range of subjects reflects the wide diversity of applications that are emerging in research on ancient DNA, including the study of DNA to analyze kinship, recovery of DNA from organisms trapped in amber, ancient DNA from human remains preserved in a variety of locations and conditions, DNA recovered from herbarium and museum specimens, and DNA isolated from ancient plant seeds or compression fossils. Ancient DNA will serve as a valuable source of information, ideas, and protocols for anyone interested in this extraordinary field. |
Contents
| 1 | |
DNA Fingerprinting | 13 |
Evolutionary Analysis | 31 |
General Aspects of Sample Preparation | 59 |
Fixed and Embedded Samples | 69 |
DNA from Amber Inclusions | 92 |
Wet Samples | 104 |
Frozen Samples | 122 |
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Ancient DNA: Recovery and Analysis of Genetic Material from Paleontological ... Bernd Herrmann,Susanne Hummel No preview available - 1993 |
Common terms and phrases
aDNA agarose gel alleles amber ancient DNA applications biological bone buffer burial cells centrifugation Chelex chromosome cloning contamination d-loop damage degradation denaturation detection digestion DNA extraction DNA polymerase DNA sequences EDTA electrophoresis embedded enzyme Epplen JT estimate ethanol ethidium bromide evolution evolutionary extracted DNA fingerprinting forensic fossil DNA fragments gene genetic genome genotypes Golenberg Hagelberg herbarium specimens Higuchi Huari human DNA hybridization hypervariable laboratory lane loci maize material method mitochondrial DNA modern molecular weight molecules mtDNA mummified mummy museum mutations nuclear nucleic acids oligonucleotide Pääbo Pathol pathology PCR amplification PCR products phylogenetic phylogeny plant Poinar polymerase chain reaction polymorphism population preserved primers probes procedure protein proteinase K protocol ratite rDNA Rollo samples seeds sequence analysis soft tissue solution Southern blot species stained studies Taq polymerase target techniques temperature template tion trees tubes variation Wilson AC Windover